Terminal Deoxynucleotidyl Transferase antibody (TdT) is a nuclear protein widely used as a marker for lymphoblastic leukemia. TdT is a template-independent DNA polymerase which has been shown to be responsible for the addition of nucleotides at the N-region junction of rearranged Ig heavy chain and T-cell receptor gene segments during the maturation of B- and T-cells. Studies have shown that a panel of antibodies consisting of TdT, CD10, CD99 (MIC2), Bcl-2 and CD34 can be used to distinguish lymphoblastic leukemias from small noncleaved cell lymphomas.
1. Orazi A, Cotton J, Cattoretti G et al. Terminal deoxynucleotidyl transferase staining
in acute leukemia and normal bone marrow in routinely processed paraffin sections. Am J Clin Pathol 1994 Nov;102(5):640-5
2. Pileri SA et al. Acute leukaemia immunophenotyping in bone-marrow routine sections. . Br J Haematol 1999 May;105(2):394-401.
3. Soslow RA, Bhargava V, Warnke RA. MIC2, TdT, bcl-2, and CD34 expression in paraffin-embedded high-gradelymphoma/acute lymphoblastic leukemia distinguishes between distinct clinicopathologic entities. Hum Pathol 1997 Oct;28(10):1158-65.
4. Center for Disease Control Manual. Guide: Safety Management, NO. CDC-22, Atlanta, GA. April 30, 1976 “Decontamination of Laboratory Sink Drains to Remove Azide Salts.”
5. National Committee for Clinical Laboratory Standards (NCCLS). Protection of laboratory workers from infectious diseases transmitted by blood and tissue; proposed guideline. Villanova, PA 1991;7(9). Order code M29-P.