Programmed death 1 (PD-1) is a cell surface co-receptor member of the CD28/CTLA-4 family, and functions as a downregulator of the immune system through a dual mechanism of inhibition (1). PD-1 is expressed on the cell surface of activated T- and B-cells. Anti-tumor immunity may be controlled by the PD-1/PD-L1 signaling pathway. PD-L1, one of the ligands associated with PD-1, provides immunity for tumor cells by inducing apoptosis of activated T cells or by inhibiting cytotoxic T cells (1,2). Therapies that target the PD-1 receptor have shown unprecedented results with high levels of clinical response in patients with various cancer types (2,3). The presence of PD-1 positive tumorinfiltrating lymphocytes (TIL) has been associated with poor prognosis in human breast cancers and may be useful in antibody therapy targeting the PD-1/PD-L1 signaling pathway (1). Treatments targeting PD-1 and its ligand, PD-L1, have also shown encouraging results in melanoma, non-small-cell lung cancer, and renal cell carcinoma (3-5). This antibody can also be used in multiplex stains with other antibodies such as CD4, CD8, FOXP3, cytokeratin and melanoma markers (6).
|VOLUME||0.1 ml, 0.5 ml, 6.0 ml|
|SPECIES REACTIVITY||Human; others not tested|
|ANTIGEN||Synthetic peptide derived from a region of the PD-1 protein|
1. Muenst S, et al. The presence of programmed death 1 (PD-1)-positive tumor-infiltrating lymphocytes is associated with poor prognosis inhuman breast cancer. Breast Cancer Res Treat. 2013 Jun; 139(3):667-76.
2. Kim JW, Eder JP. Prospects for Targeting PD-1 and PD-L1 in Various Tumor Types. Oncology. (Williston Park). 2014 Nov; 28(11 Suppl 3).
3. Tumeh PC, et al. PD-1 blockade induces responses by inhibiting adaptive immune resistance. Nature. 2014 Nov 27; 515(7528):568-71.
4. D’Incecco A, et al. PD-1 and PD-L1 expression in molecularly selected non-small cell lung cancer patients. Br J Cancer. 2015 Jan 6; 112(1):95-102.
5. Tykodi SS. PD-1 as an emerging therapeutic target in renal cell carcinoma: current evidence. Onco Targets Ther. 2014 Jul 25; 7:1349-59.
6. Yang G, et al. A multiplex IHC evaluation of multiple immune checkpoint receptors and mismatch repair proteins in colorectal carcinoma [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2018; 2018 Apr 14-18; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2018;78(13 Suppl):Abstract nr 1026.
7. Center for Disease Control Manual. Guide: Safety Management, NO. CDC-22, Atlanta, GA. April 30, 1976 “Decontamination of Laboratory Sink Drains to Remove Azide Salts.”
8. Clinical and Laboratory Standards Institute (CLSI). Protection of Laboratory Workers from Occupationally Acquired Infections; Approved Guideline-Fourth Edition CLSI document M29-A4 Wayne, PA 2014.