H3K27me3 (histone 3 lysine 27 trimethylation) is an epigenetic mark that plays a critical role in regulation of gene expression. The dysregulation of H3K27me3 is implicated in the genesis and progression of cancer. Studies indicate that H3K27me3 plays a role in the creation and maintenance of cell type-specific programs of transcriptional control for a wide variety of species and cell fates.1 H3K27me3 expression was shown to be a significant prognostic indicator in breast, ovarian and pancreatic cancers. Low expression of H3K27me3 correlated with significantly shorter overall survival time compared to individuals with high H3K27me3 expression.2 H3K27me3 may be a useful marker in diagnosing malignant peripheral nerve sheath tumors (MPNSTs) and providing molecular insight in progression of prostate cancer.3,4
1. Arthur RK, et al. Evolution of H3K27me3-marked chromatin is linked to gene expression evolution and to patterns of gene duplication and diversification. Genome Res. 2014 July; 24:1115-24.
2. Wei Y, et al. Loss of trimethylation at lysine 27 of histone H3 is a predictor of poor outcome in breast, ovarian, and pancreatic cancers. Mol Carcinog. 2008 Sep; 47(9):701-06.
3. Prieto-Granada CN, et al. Loss of H3K27me3 Expression Is a Highly Sensitive Marker for Sporadic and Radiation induced MPNST. Am J Surg Pathol. 2016 Apr; 40(4):479-89.
4. Ngollo M, et al. Global Analysis of H3K27me3 as an Epigenetic Marker in Prostate Cancer Progression. BMC Cancer. 2017; 17:261.
5. Center for Disease Control Manual. Guide: Safety Management, NO. CDC-22, Atlanta, GA. April 30, 1976 “Decontamination of Laboratory Sink Drains to Remove Azide Salts.”
6. Clinical and Laboratory Standards Institute (CLSI). Protection of Laboratory Workers from Occupationally Acquired Infections; Approved Guideline-Fourth Edition CLSI document M29-A4 Wayne, PA 2014.